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. 2015 Sep 8;11(9):e1005508. doi: 10.1371/journal.pgen.1005508

Fig 3. Reduced or absent N-DRC and other axonemal proteins are not restored in pf7; pf8 suppressors.

Fig 3

Ten micrograms of axonemes from various strains were used in the immunoblots. (A) Strains resuspended in nitrogen-free medium for 4 hours at 21°C. TG, transgene. (B) Cells were resuspended in nitrogen-free medium for 2 hours at 21°C. One-half of cells from each strain were switched to 32°C for 4 hours while the other half was maintained at 21°C for 4 hours. α-tubulin is included as a loading control.