Table 2. The membrane fatty acid profile of L. monocytogenes parent strain 10403S and the BCFA-deficient mutant MOR401 grown in BHI at 37ᵒC with or without BCCA precursors.
% (wt/wt) of total fatty acids
| Strain, growth conditions |
Anteiso odd | Iso odd |
Iso even |
Straight even |
Novel fatty acids | BCFA | SCFA | ||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| C15:0 | C17:0 | SUM | |||||||||
| 10403S | 47.77±0. 59 | 34.76±0. 4 | 82.5 | 11.7 | 3.82 | 1.94 | ND | ND | ND | 98.07 | 1.94 |
| MOR401 | 5.4±1.9 | 2±1.8 | 7.4 | 1.6 | 24.1 | 57.2 | ND | ND | ND | 33.2 | 66.0 |
| MOR401+2 MB | 52.3±1.5 | 40±0.7 | 93.1 | ND | 0.74 | 6.2 | ND | ND | ND | 93.8 | 6.2 |
| MOR401+2EB | 0.6±0.01 | 1.8±0.3 | 2.3 | ND | 2.39 | 12.4 | ND | 12-ethyltetradecanoic acid 50.4±2.7 | 14-ethylhexadecanoic acid 31.6±0.6 | 86.8 | 13.3 |
| MOR401+2 MP | 1.4±0.5 | 2.4±0.7 | 3.8 | ND | 10.5 | 5.4 | 10-methyltridecanoic acid 1.04±0.08 | 12-methylpentadecanoic acid 64.5±0.6 | 14-methylheptadecanoic acid 12.3±2.6 | 92.4 | 6.8 |
| MOR401+trimethylacetate | 7.6±1.3 | 10.2±0.1 | 17.8 | ND | 28.1 | 11.2 | ND | 12-dimethyltridecanoic acid 21.1±1.4 | 14-dimethylpentadecanoic acid 20.2±1.4 | 87.2 | 11.2 |
| MOR401+2,2-dimethylbut yrate | 9.5±1.5 | 12.02±0. 01 | 21.5 | ND | 35.3 | 10.8 | ND | 12,12-dimethyltetradecanoic acid 21.5±1.6 | 14,14-dimethylhexadecanoic acid 8±0.6 | 86.3 | 13.7 |
All supplements were used at 1 mM.
The percentages of respective fatty acids are means from three independent experiments with standard deviations
ND- Not detected
The minor fatty acids (<1%) are not reported. This includes minor percentages of odd numbered SCFAs in some cases.