Table 3. The membrane fatty acid profile of L. monocytogenes parent strain 10403S and the BCFA-deficient mutant MOR401 supplemented with BCCA precursors grown in BHI at 10°C.
% (wt/wt) of total fatty acids
| Strain, growth conditions |
Anteiso odd | Iso odd |
Iso even |
Novel fatty acids | BCFA | SCFA | ||||
|---|---|---|---|---|---|---|---|---|---|---|
| C15:0 | C17:0 | SUM | ||||||||
| 10403S | 69.9±1.5 | 7.6±0.8 | 78.6 | 15.2 | 4.9 | ND | ND | ND | 98.8 | 0.6 |
| MOR401+2MB | 77.9±1.4 | 15.8±0.5 | 94.7 | ND | 0.1 | ND | ND | ND | 95.3 | 3.6 |
| MOR401+ 2EB | 1.4±0.21 | 1.5±0.7 | 2.9 | ND | 4.3 | ND | 12-ethyltetradecanoic acid 65.9±5.5 | 14-ethylhexadecanoic acid 14.2±2.6 | 87.7 | 10.8 |
| MOR401+2MP | 1.7±0.18 | 0.6±0.8 | 2.3 | ND | 11.8 | 10-methyltridecanoic acid 14.4±5 | 12-methylpentadecanoic acid 65.9±0.42 | 14-methylheptadecanoic acid 0.5±0.63 | 94.2 | 4.5 |
All supplements were used at 1 mM.
The percentages of respective fatty acids are means from three independent experiments with standard deviations
ND- Not detected
The minor fatty acids (<1%) are not reported.