Table 1. RT-qPCR primer sets used in this study and their target genes.
| Gene name | Gene ID | Primer name | Forward primer (5’-3’) | Reverse primer (5’-3’) |
|---|---|---|---|---|
| Bic-C1 | Glyma.08G349900 | Bic-C1 | GTATGCTGATCGGGTGGAGA | AGCTGGGGTTCATCATCCTC |
| Bic-C2 | Glyma.03G064800 | Bic-C2 | GCCTTAATGATGTGAATGGT | AGAGATCATGTTCCCACTTG |
| F-box protein2 | Glyma.02G273700 | F-box protein2 | TGAGAAAGCTGTTGAGGATT | GATTGCTCTTAAATCCATGC |
| GPX | Glyma.08G013800 | GPX | TCTCTATTTCAGCCACTCGT | GGTTTGAAGGAAGTGTGAAG |
| RHA | Glyma.08G113000 | RHA | ACTGGTAGATTTGCTGGAGA | CTTATTTGTGGCTCAAAACC |
| SF | Glyma.05G117600 | SF | CTCTCTTCGACAAGTATGGG | CAAACTGGACCGTTATTTCG |
| VPS-like | Glyma.09G196600 | VPS-like | AAAGAGTCTCATCCCACAAC | CGCATATTCCCAATCTCAGA |
| ACT11 * | Glyma.18G290800; Glyma.02G091900 | ACT11.C | ATTTTGACTGAGCGTGGTTATTCC | GCTGGTCCTGGCTGTCTCC |
| ACT2/7 * | Glyma.19G147900 | ACT2/7.A | CTTCCCTCAGCACCTTCCAA | GGTCCAGCTTTCACACTCCAT |
| CYP * | Glyma.12G024700 | CYP.B | ACGACGAAGACGGAGTGG | CGACGACGACAGGCTTGG |
| ELF1a * | Glyma.05G114900; Glyma.19G052400 | ELF1a | GACCTTCTTCGTTTCTCGCA | CGAACCTCTCAATCACACGC |
| ELF1b * | Glyma.02G276600; Glyma.14G039100 | ELF1b.B | CCACTGCTGAAGAAGATGATGATG | AAGGACAGAAGACTTGCCACTC |
| G6PD * | Glyma.16G063200 | G6PD | ACTCCTTGATACCGTTGTCCAT | GTTTGTTATCCGCCTACAGCCT |
| TUB4 * | Glyma.03G124400; Glyma.19G127700 | TUB4.B | TGGCGTCCACATTCATTG | GAACTCCATCTCGTCCAT |
| UBQ10 * | Glyma.07G199900 | UBQ10 | TCCCACCAGACCAGCAGAG | CACGAAGACGCAACACAAGG |
* Commonly used reference genes.