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. 2015 Sep 3;10:5635–5646. doi: 10.2147/IJN.S89910

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© 2015 Wang et al. This work is published by Dove Medical Press Limited, and licensed under Creative Commons Attribution – Non Commercial (unported, v3.0) License

The full terms of the License are available at http://creativecommons.org/licenses/by-nc/3.0/. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed.

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Confocal microscopic images of C2C12 cells treated with FITC-PMO (2 µg) without and with PE-4 (5 µg). Nuclear staining with Hoechst 33258. The images were obtained under a magnification of 63×. Labeled PMO can be observed at lower levels throughout the treated cells; however, with the addition of PE-4, greater levels of labeled PMO can be observed with greater concentrations in the perinuclear space.

Abbreviations: PE, polyelectrolyte; FITC, fluorescein isothiocyanate; PMO, phosphorodiamidate morpholino oligomer.