Skip to main content
. Author manuscript; available in PMC: 2015 Oct 5.
Published in final edited form as: Brain Res. 2015 Jun 20;1622:7–21. doi: 10.1016/j.brainres.2015.06.014

Fig. 3. CIE-induced loss of axonal NFPs and elevation of deNFP in brain and spinal cord.

Fig. 3

Double or single immunofluorescent staining was performed in coronal spinal cord slices and longitudinal brain slices to illustrate corpus callosum. Images were captured at 200 × magnification; n = 3-4. (A) Immunoblot and corresponding bar graphs (% change, naïve taken as 100%) showed reduction in NFL (68 kDa) in hippocampus in CIE 24 h compared with naive (*p < 0.0001; n = 4-6). Blots were re-probed for β-actin (42 kDa). Immunofluorescent images (lower panel) indicated substantially reduced levels of NFL (red) and NFH (green) IR in spinal cord in CIE 24 h (withdrawal) compared with control. (B) Immunofluorescent images showed elevated levels of deNFP in spinal cord, corpus callosum and cerebellum, indicating significant axonal degeneration in CIE 24 h compared to control.