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. Author manuscript; available in PMC: 2016 Oct 5.
Published in final edited form as: Brain Res. 2015 Jul 2;1622:240–251. doi: 10.1016/j.brainres.2015.06.030

Figure 3. Comparison of oxygen consumption rates (OCR) and extracellular acidification rates (ECAR) in synaptosomal mitochondria derived from the striatum (ST) and mediobasal hypothalamus (MBH).

Figure 3

OCR and ECAR measurements were performed using a Seahorse XF24 analyzer, with 20 μg of synaptosomal protein derived from each region yielding a respiratory control ratio' (RCR') of greater than 3. A; OCR (pmoles/min/μg) of ST- and MBH-derived synaptosomal mitochondria during basal, maximum and spare aerobic respiration measured using a Seahorse XF analyzer. Columns represent mean OCR of synaptosomal mitochondria and vertical lines + 1 SEM of determinations in 5 mice. * indicates OCR of maximum and spare respiration in MBH that are significantly different (p < 0.05) from those in ST. B; ECAR (mpH/min/μg) of ST- and MBH-derived synaptosomal mitochondria under basal conditions, and following inhibition (rotenone) or uncoupling (FCCP) of the mitochondrial electron transport chain. * indicates ECAR in FCCP- and rotenone-treated groups that are significantly different (p < 0.05) from basal ECAR values for each region.