Figure 1.

TLR costimulation does not augment T‐cell activation, expansion, and differentiation. Wild‐type C57BL6 mice received an adoptive transfer of 10⁵ CD4⁺ purified, naïve 1807 Tg cells and were infected with 2 × 10⁶ live F. pedrosoi spores or not. Transferred 1807 and endogenous CD4⁺ T cells were harvested from the popliteal LN and the (A) number and (E) frequencies of activated (CD44⁺), and (B) number of IL‐17‐ and IFN‐γ‐producing T cells was assessed at day 7 postinjection by flow cytometry. (C) Dot plots show concatenated samples of 4–6 mice/group. The numbers indicate the mean ± SEM of activated (CD44^hi) and cytokine producing 1807 Tg (Thy1.1⁺) cells and endogenous CD4⁺ T cells. (D and E) The frequencies of cytokine‐producing 1807 cells were assessed by flow cytometry. Data are expressed as the mean ± SEM (n = 4–6 mice/group). T‐cell numbers from LPS or Imiquimod‐treated, infected mice versus nontreated infected mice were not statistically different using the Wilcoxon rank test for nonparametric data. Data are from single experiments representative of three independent experiments.