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. 2015 Jul 28;45(9):2542–2552. doi: 10.1002/eji.201545591

Figure 2.

Figure 2

F. pedrosoi spores trigger Dectin‐1 and Dectin‐2 signaling and IL‐6 production. (A) BWZ cells and a subline expressing Dectin‐1‐CD3ζ (Dectin‐1), as well as B3Z cells expressing Dectin‐2, Mincle, FcRγ chain, Dectin‐2 + FcRγ, MCL + FcRγ, Dectin‐2 + MCL + FcRγ, Mincle + FcRγ or Mincle + MCL + FcRγ were stimulated with live F. pedrosoi spores. After 18 h, lacZ activity was measured using a colorimetric assay and expressed as OD 560/620 values. Data are expressed as the mean ± SD of duplicate wells. Data are from single experiments representative of three independent experiments. (B) Bone marrow derived dendritic cells (BMDC) were cocultured with live F. pedrosoi spores for 24 h and IL‐6 was detected in the supernatants by ELISA. Numbers above histogram bars indicate the n‐fold change versus the corresponding wild‐type control. Data are from a single experiment representative of two independent experiments.