Figure 4.

F. pedrosoi‐induced T‐cell differentiation requires Dectin‐2, but is suppressed by Mincle. Wild type, Myd88^−/−, Card9^−/−, FcRγ^−/−, Dectin‐1^−/−, Dectin‐2^−/−, Mincle^−/−, and Clec4d^+/+ and Clec4d^−/− mice adoptively received 10⁵ CD4⁺ purified, naïve 1807 Tg cells and were infected with 2 × 10⁶ live F. pedrosoi spores or not. At day 7 postinfection, the popliteal lymph nodes were harvested and (A, B) the number and (C) frequencies of (A) IL‐17‐ and (B) IFN‐γ‐producing T cells enumerated by flow cytometry. The values over the bars in the histogram indicated the n‐fold change in T‐cell numbers versus the corresponding wild‐type control mice. The dot plots show the sum of concatenated events from five mice per group and the numbers indicate the mean of endogenous and 1807 CD4⁺ T cells. Data are expressed as mean + SD of five mice per group from a single experiment representative of two independent experiments. *p < 0.05 versus infected wild‐type controls using the Wilcoxon rank test for nonparametric data.