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. 2015 Aug 24;8(4):318–326. doi: 10.1016/j.tranon.2015.06.004

Figure 4.

Figure 4

Correlation between cytokine concentrations in supernatant of three-dimensionally cultured breast cancer tissues and WST-8 assay values of the cultured tissues. The growth abilities of the tissues three-dimensionally cultured in TGP gel were assessed by WST-8 assay (absorbance at 450 nm) after the culture. Correlations were evaluated by Pearson correlation coefficient test and Spearman correlation coefficient test (n = 21). Concentrations for IFN-γ (A; P = 0.0031 by Pearson), IL-6 (D; P = 0.0274 by Pearson and P = 0.0009 by Spearman), and IL-10 (E; P = 0.0158 by Pearson and P = 0.0003 by Spearman) in the culture supernatant were significantly correlated with the growth abilities of the tissues as evaluated by the WST-8 assay, whereas those of TNF-α (B), IL-2 (C), and IL-17A (F) were not.