Table 3.
Relationship between ER or PgR Expression Incidence and the WST-8 Assay Value (Index for Tumor Growth) of Three-Dimensionally Cultured Breast Cancer Tissues
| WST-8 Assay Value for 3D Cultured Sample |
||||
|---|---|---|---|---|
| Higher | Lower | Total | ||
| ER | + | 5 | 8 | 13 |
| − | 6⁎ | 2 | 8 | |
| Total | 11 | 10 | 21 | |
| PgR | + | 3 | 7 | 10 |
| − | 8 | 3 | 11 | |
| Total | 11 | 10 | 21 | |
The patients were grouped into the higher and lower subjects expressing higher and lower WST-8 assay values, respectively, by the median score (0.3026 read by absorbance at 450 nm) for the results of WST-8 assay of 3D cultured sample. On the other hand, the patients were divided into the ER positive (+) and ER negative (−) or PgR positive (+) and PgR negative (−) groups. ER and PgR of patients’ cancer tissue were detected by immunohistochemical analysis. Statistical analysis for the incidence of subjects with cancer tissues exhibiting the high WST-8 values in vitro was performed by χ2 test. The ER(−) tissues exhibited a significantly high incidence of the greater WST-8 assay-values (⁎P = 0.0306).