Figure 2.

Ins1Cre expression results in excision of the flox'd region of LepRb alleles selectively in islets. Genomic DNA was harvested from Ins1CreLepR^KO and control LepR^F/F animals and used as a template for PCR with the primers indicated (A). Predicted product sizes are 339 bp for the flox'd allele (primers b,c) and 207 bp for the excised allele (primers a,c) (B). Ins1CreLepR^KO animals displayed a normal progression in bodyweight. Ins1CreLepR^KO males (green line) and LepR^F/F (black line) and below Ins1CreLepR^KO females (red line) and LepR^F/F (black line). (C). Blood glucose concentration after overnight fast in males (D) (Ins1CreLepR^KO, n = 8, LepR^F/F, n = 7) and females at 8 weeks (E) (Ins1CreLepR^KO, n = 13, LepR^F/F, n = 9). Plasma insulin levels in male mice (F) Ins1CreLepR^KO, n = 10, LepR^F/F n = 7, and in females (G) Ins1CreLepR^KO, n = 9, LepR^F/F, n = 7. β to α cell ratio in Ins1CreLepR^KO and wild-type mice (H).