Figure 5.

Ins1CreLepR^KOislets display impaired Ca²⁺responses to high glucose. Ca²⁺ recordings from control LepR^F/F islets (black line) and Ins1CreLepR^KO islets (red line) in response to either 11 mM glucose (A). Inset are area under the curve (AUC) and amplitudes (ΔF (F_max-F_min)) of the Ca²⁺ rises. Data are from 14 to 16 islets (3–4 mice) per genotype. (B,C) Connectivity analysis [44] showing the proportion (%) of significantly correlated cell pairs during a step change from 3 to 11 mM glucose (i.e. the activity onsets), or at steady state in the continued presence of 11 mM glucose. Pseudocolor plots in (C) show the strength of connections, determined using by Pearson Correlation (Pearson R). Data are expressed as mean ± SEM. Statistical analysis was performed using two-way ANOVA and Student's t-test. *P < 0.05.