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. 2015 Aug 26;2015:293524. doi: 10.1155/2015/293524

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Copyright © 2015 Judith M. Ball et al.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Western blot analysis of HT29.f8 cell lysates. Five micrograms of HT29.f8 cell lysates was separated on a 12.5% SDS-PAGE, electroblotted onto nitrocellulose membranes, probed with rabbit anti-NSP4_150–175 peptide-specific and goat anti-rabbit HRP-conjugated IgG, and visualized with Super Signal West Pico chemiluminescent substrate (Pierce) followed by exposure to Kodak X-OMAT film. (Lane 1) RV-infected HT29.f8 cells and (Lane 2) RV-infected HT29.f8 cells with 0.02% DMSO, respectively, show cleavage fragments and unglycosylated, mono-, diglycosylated, and multimeric forms of NSP4. (Lane 3) RV-infected HT29.f8 cells with 20 μM t-A1 and (Lane 4) RV-infected HT29.f8 cells with 20 μM t-A3 only show the diglycosylated form of NSP4. (Lane 5) HT29.f8 with no virus showed NSP4 banding pattern.