Table 3.
IFN-γ production against overlapping peptides of Rv2626c by PBMCs from non-TB individuals.
| pg/ml of IFN-γ, Mean ± SEM |
||||||||
|---|---|---|---|---|---|---|---|---|
| N | Pool A | Pool B | Pool C | Pool D | Pool E | Pool F | Cumulative response to pools B, D and F | |
| HD | 33 | 7.6 ± 4.09 | 3.9 ± 1.6 | 4.1 ± 6.0 | 18.4 ± 18.4 | 4.9 ± 3.4 | 34.4 ± 20.0 | 55.3 ± 20.92 |
| LTBI | 40 | 216.6 ± 82.7 | 275.9 ± 93.1 | 119.2 ± 35.7 | 416.2 ± 120.3 | 48.7 ± 20.7 | 517.1 ± 119.3 | 1177.0 ± 253.1 |
| p value | 0.09 | < 0.001 | 0.03 | < 0.001 | 0.02 | < 0.001 | < 0.001 | |
Peripheral blood mononuclear cells (PBMCs) from healthy donors (HD) and latently M. tuberculosis infected individuals (LTBI) were cultured with six peptide pools (A–F) derived from Rv2626c (5 μg/ml of each peptide) for five days and then IFN-γ production was evaluated in cell free supernatants by ELISA. The cumulative response to pools B, D and F was calculated for each individual as the sum of the secretion of IFN-γ in response to each of the three pools. p values were obtained though the Mann–Whitney test for unpaired samples.