Figure 6. Phosphokinase and cathepsin signatures of freshly isolated monocytes from breast cancer patients were predicted using model trained on macrophage-assisted invasion.

Whole blood was collected from breast cancer patients (BCP) undergoing surgery (lumpectomy, mastectomy), and monocytes were freshly isolated and lysed for (A) phosphokinase signatures and cathepsin activity with a representative cathepsin zymogram shown for four patients and cropped for space, but shows all cathepsin active bands per patient. These data were then input into the trained PLSR model to calculate the outcomes of the monocyte-derived macrophage-assisted invasion and cystatin C levels after differentiation to determine if blood signatures would be useful to predict patient invasion outcomes. (B) The loadings plot is shown and unique breast cancer patients co-vary with patients from the trained model with highest invasion of #4 down to #3 with lowest invasion to indicate possible phenotypes of the MDMs from the women with breast cancer.