Figure 3. Defective regional decidualization with abnormal cell cycle activity results from the loss of uterine FoxM1.

(A) (a,b), Histological analyses by hematoxylin and eosin staining of D8-IS between null (FoxM1^d/d) and control (FoxM1^f/f) mice. Mesometrial (M), lateral (L) and antimesometrial (AM) locations are demarcated by broken lines. (c), A representative IS shows signs of resorption for null mice. (B) Comparison of developmental area (%) for M and SDZ (L+AM) locations on D7-8 IS between null and control mice. *Significantly different (p < 0.001) for corresponding M or SDZ locations between null and control on particular day of pregnancy. (C) Immunofluorescence analyses of BrdU and pHH3 at D7-IS between null and control. (D) Quantitative analyses of BrdU or pHH3 positively stained cells (%) in M and SDZ locations of D7-8 IS between null and control mice. *Significantly different (p < 0.001) between null vs. control. (E) Quantitative RT-PCR analyses of cell cycle regulatory genes [Ccnb1, Cdc25b, Cenpf, and Cdkn1a (p21)] at D7-IS for control and null mice. **Significantly different (p < 0.001) between null vs. control. (F) A representative flow cytometric analysis of cell cycle distribution based on DNA content for decidual cells isolated on D8-IS between null vs. control. (G) Cellular distribution (%) for 2N, 4N, and >4N populations at the D8-IS between null vs. control. *Significantly different (p < 0.001) for corresponding groups between control vs null mice.