Figure 4. FoxM1 deficiency in stromal cells leads to enhanced cell cycle arrest prior to mitosis with aberrantly upregulated polyploidy during decidualization in vitro.
(A) A representative flow cytometric analysis of cell cycle distribution based on DNA content for cells after in vitro decidualization for 3-days between null (FoxM1d/d) vs. control (FoxM1f/f) stromal cells. (B) Cellular distribution (%) for 4N and >4N populations after in vitro decidualization at indicated days (1–7 days) between null vs. control cells. **p < 0.001 and *p < 0.05. (C) Quantitative RT-PCR analyses of FoxM1, Ccnb1, Cdc25b, Cenpf, Bmp2, and Prl8a2 after in vitro decidualization for 3 days between null and control cells. **p < 0.001 and *p < 0.05. (D) Cell cycle phase-specific count (%) in S and G2/M after the release in cell cycle for proliferation in vitro at indicated times (0–24 h) between null and control cells. **p < 0.001 and *p < 0.05. E. Quantitative analysis of pHH3-positive cell count (%) after in vitro decidualization at indicated times (0 and 24 h) between null and control cells. *p < 0.05. F. Analysis of Cyclin B1 protein after in vitro decidualization at indicated times (0 and 24 h) between null and control cells. *p < 0.001.