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. 2015 Sep 9;5:13819. doi: 10.1038/srep13819

Figure 1. qRT-PCR analysis of OsABA8ox1, OsABA8ox2, OsABA8ox3, and Rab16A under stress conditions and hormone treatments.

Figure 1

One-week-old seedlings of rice were used for these experiments. For drought treatment (Dry), seedlings were dehydrated on a paper towel. For hormone or osmotic stress treatment, plants were transferred to buffer containing 100 μM abscisic acid (ABA), 100 μM salicylic acid (SA), 100 μM jasmonic acid (JA), 100 μM ethephon (Eth), 250 mM NaCl, or 500 mM mannitol. For cold treatment, plants were transferred to and kept at 4 °C for 6 h or 24 h. For wounding, shoots were punctured by a needle and grown hydroponically in the buffer for 6 or 24 h. Gene expression values are means (±SD) of three biological replicates. Significant differences relative to controls were evaluated using Student’s t-test. *, **, and *** represent P < 0.05, P < 0.01, and P < 0.001, respectively.