Table 2. List of differentially expressed proteins of P. indica co-cultured with A. chroococcum M4, as determined by 2-DE and MALDI-TOF/MS.
| Spot Noa | Spot identifier number b | Protein name (Accession No)c | Organismd | Scoree | MW/PIf | Sequence Coverage (%)g | No of peptides matchedh | Role in biological process | P valuei | Fold changej |
|---|---|---|---|---|---|---|---|---|---|---|
| 17Fc | 334 | Hypothetical protein PIIN_05768 _gi|353239942 | P. indica | 31 | 36.860/9.65 | 8.0 | 4 | A nickel dependent regulatory enzyme involved in recycling of urea. | 1.079e-005 | 2.9 ↓ |
| 18Fc | 320 | u3 small nucleolar RNA-associated protein 11_ gi|299756136 | C. cinerea | 25 | 39.845/9.30 | 4.1 | 3 | Probably function as small nucleolar ribonucleoproteins in pre-ribosomal RNA processing reactions or in other biological function. | 3.589e-004 | 1.5 ↓ |
| 19Fc | 341 | related to RTM1 protein_gi|353241549 | P. indica | 37 | 33.056/10.0 | 9.5 | 2 | Provide immunity and resistance to the fungus againstenvironmental cytotoxic compounds. | 1.306e-007 | 5.1 ↓ |
| 21Fc | 292 | Probable ENO1-enolase I (2-phosphoglycerate dehydratase )_gi|353244894 | P. indica | 98 | 43.573/5.3 | 23.7 | 6 | Key regulatory enzyme that participates in glycolytic pathway for generating reducing powers for ATP synthesis. | 2.505e-004 | 3.0 ↓ |
| 4M | 452 | hypothetical protein PIIN_04261_ gi|353238374 | P. indica | 20 | 22.836/9.10 | 15.2 | 2 | NA | 0.141 | 1.9* |
| 5M | 422 | G protein alpha-subunit _ gi|170108471 | Laccaria bicolor | 68 | 8.129/4.6 | 47.8 | 3 | GTP binding protein,probably involved in signaling for cell growth. | 0.235 | 1.5* |
| 12M | 331 | predicted protein_ gi|170098885 | Laccaria bicolor | 53 | 33.900/6.1 | 17.5 | 3 | NA | 0.133 | 1.3* |
| 13Mu | 309 | predicted protein _gi|170085531 | Laccaria bicolor | 25 | 36.625/10.1 | 4.2 | 3 | NA | 7.660e-006 | 1.5 ↑ |
| 20Mu | 301 | Hypothetical protein PIIN_01661_ gi|353235830 | P. indica | 58 | 39.858/10.0 | 20.0 | 3 | Mitotic chromosome segregation. | 0.005 | 1.3 ↑ |
| 22Mu | 210 | Hypothetical protein PIIN_01521_ gi|353235685 | P. indica | 49 | 44.690/5.8 | 10.4 | 3 | Involved in the process of signaling path way that regulate cell division. | 0.001 | 1.5 ↑ |
| 23M | 198 | Hypothetical protein PIIN_03309 _gi|353237436 | P. indica | 45 | 57.965/6.80 | 11.4 | 3 | NA | 0.466 | 1.5* |
aSpot numbers refer to the numbers on the 2DE gels shown in Fig. 5A,C.
bProtein spot identifier number according to the progenesis.
cProtein accession number according to the MASCOT software.
d,e,f,g,hName of the organism, score, theoretical MW/PI, sequence coverage, no of peptides matched according to the MASCOT software.
eP value according to progenesis samespots analysis.
fProtein fold change according to the 2D-PAGE analysis of progenesis samespots analysis software shown in Fig. S3. ‘Fc’ represents Fungus control, ‘Mu’ represents upregulated and Md represents downregulated fungus treated with M4. ‘↑’ and ‘↓’ Represents up and down regulation. *Represents no significant changes in the fold level.