Skip to main content
. 2015 Jun 15;125(7):2721–2735. doi: 10.1172/JCI64502

Figure 2. AAV-sh[SNCA] specifically targets Snca mRNA in the substantia nigra in vivo.

Figure 2

The substantia nigra of three rats was transduced with AAV-sh[SNCA] on one side and AAV-sh[control] on the other (cohort 1). Twenty-one days later, contiguous midbrain sections were labeled by RNA in situ hybridization using antisense cRNA probes to Snca, Sncb, and Sncg; by a sense control probe; or by immunofluorescence for TH to label dopaminergic neurons. (A) Low-power micrographs of the midbrain. Arrows indicate the position of the substantia nigra, and arrowheads indicate punch marks labeling the side that received AAV-sh[SNCA]. Scale bar (for all panels): 1 mm. (B) The substantia nigra on each side of the corresponding section is shown at higher magnification. Specific loss of the Snca transcript is seen after transduction with AAV-sh[SNCA] but not AAV-sh[control]. Scale bar (for all panels): 100 μm.