Cell growth was measured by manual counting. Knockdown efficiency of DAPK1 siRNA in each cell line is plotted in each panel (black). siRNA against luciferase was used as a control (gray). (A) Growth of ER-positive, p53-WT breast cancer cell lines upon DAPK1 knockdown by siRNA. MCF10A is not ER positive. (B) Growth of ER-positive, p53-mutated breast cancer cell lines upon DAPK1 knockdown. (C) Growth of ER-negative, p53-mutated breast cancer cell lines upon DAPK1 knockdown. DAPK1 knockdown and cell growth experiments were performed in triplicate, with results reported as average ± SEM. *P < 0.01; **P < 0.001, 2-tailed Student’s t test.