Figure 6. Stromal cell–derived IL-33 supports the development of JAK2^V617F-dependent MPNs in vivo.

BM cells from Scl-Cre JAK2^V617F transgenic donor mice were injected into lethally irradiated Il33^–/– or WT recipient mice. After 7 weeks, (A) percentage of GMPs in the BM and (B) frequency of neutrophils in the blood were assessed. (C) Alternatively, indicated blood parameters were evaluated every other week by CBC, starting 3 weeks after reconstitution. n = 5 mice per group. For C, 1 representative of 2 independent experiments is shown. (A and B) Standard Student’s t test, and (C) 2-way ANOVA with Bonferroni post hoc test were used. *P < 0.05; **P < 0.01; ***P < 0.001; and ****P < 0.0001.