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. 2015 Jun 22;125(7):2851–2860. doi: 10.1172/JCI79956

Figure 2. De novo DNA methyltransferase DNMT3A targets specific metabolic gene loci during β cell maturation.

Figure 2

(A) Expression profile of DNMT3A in representative pancreatic sections from WT mice at indicated ages (P1 to 6 weeks) using immunostaining for DNMT3A (red) and insulin (Ins; green). DAPI (blue) counter-stains the nuclei. (Representative from 3 independent experiments). Scale bar: 100 μm. (B) ChIP analysis showing the binding of DNMT3A to the Ldha (+1114 to +1220 bp) and Hk1 (–324 to –215 bp) loci, along with a negative-control primer pair (ctl) in the Arx locus (+1348 to +1470 bp) in sorted β cells from MIP-GFP mice at indicated ages. ChIP with IgG is shown as a negative control. n = 3 independent experiments. The error bars represent SEM. *P < 0.05, Student’s t test.