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. 2015 Jun 22;125(7):2851–2860. doi: 10.1172/JCI79956

Figure 4. β Cells lacking Dnmt3a are functionally immature.

Figure 4

(A) Plasma insulin levels in 6-week-old 3aRCY-KO mice and littermate-control 3aRCY-Het mice (fasted 6 hours) at 0 and 30 minutes after i.p. injection of 2 g/kg glucose (n = 3 for each genotype). (B) Insulin secretion (percentage of insulin content) in a dynamic islet perifusion assay comparing islets from 6-week-old 3aRCY-KO or control 3aRCY-Het littermates at 4 mM and 16 mM glucose. (C) Average insulin secretion (percentage of content) in the dynamic islet perifusion GSIS at 4 mM and 16 mM glucose, comparing 3aRCY-KO islets with control 3aRCY-Het islets from n = 3 mice aged 6 weeks. (D) Static incubation GSIS assay, comparing insulin secretion (percentage of insulin content) in islets from 3aRCY-KO mice with control 3aRCY-Het islets (n = 3 mice), at 2.8 mM glucose, 16.7 mM glucose, and 20 mM arginine. (E) Static incubation GSIS assay, comparing insulin secretion (percentage of insulin content) in islets from 6-week-old 3aRCY-KO mice with control 3aRCY-Het islets, at 2.8 mM glucose, 16.7 mM glucose, 100 μM diazoxide, and a 100 mM each of diazoxide and tolbutamide (n = 3 mice). (F) Static incubation GSIS assay, comparing insulin secretion (percentage of insulin content) in islets from 6-week-old 3aRCY-KO mice, treated either with a combination of siRNAs targeting Ldha and Hk1, or scrambled (Scr) siRNA, at 2.8 mM glucose and 16.7 mM glucose (n = 3 mice, islets from each mice divided into the 2 experimental groups). Error bars indicate ± SEM; *P < 0.05, **P < 0.01, ***P < 0.005, Student’s t test.