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. 2015 Jun 15;125(7):2772–2780. doi: 10.1172/JCI80705

Figure 1. Schematic of SOD1 isolation and mass spectrometry detection method.

Figure 1

(A) Flow chart detailing the isolation, processing, and detection of leucine-containing SOD1 peptides. GluC, endoproteinase Glu-C; LC-MS, LC/tandem MS. (B) Schematic representing LC/tandem MS detection of 13C6-leucine–containing peptides. The +6-Da shift in the leucine-containing peptide KADDLGKGGNEE reflects the incorporation of 13C6-leucine. (C–E) LC/tandem MS standard curves for the 3 leucine-containing peptides used in quantifying labeled SOD1.