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. 2015 Jun 22;125(7):2861–2876. doi: 10.1172/JCI80941

Figure 10. Effects of selective activation of MeA SIM1 neurons.

Figure 10

(A) mCherry immunoreactivity in the MeA of Sim1-Cre mice receiving AAV-hM3Dq-mCherry infection. (B) c-FOS immunoreactivity in Sim1-Cre mice receiving AAV-hM3Dq-mCherry infection and CNO injection (3 mg/kg, i.p.). (C) c-FOS immunoreactivity in WT mice receiving AAV-hM3Dq-mCherry infection and CNO injection (3 mg/kg, i.p.). (D) c-FOS immunoreactivity in Sim1-Cre mice receiving AAV-hM3Dq-mCherry infection and saline injection. 3V, 3rd ventricle; opt, optic tract. (E–H) AAV-hM3Dq-mCherry-infected Sim1-Cre mice received i.p. injections of saline or CNO (3 mg/kg) at 9 am. Physical activity (E) and body temperature (F) were measured. (G) Food intake during 2 hours after i.p. injections in mice. (H) Physical activity within 30-minute bin was monitored for up to 7 hours after i.p. injections in mice. Data are presented as mean ± SEM. n = 6/group. *P < 0.05 in t tests.