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. 2015 May 26;125(7):2626–2630. doi: 10.1172/JCI81070

Figure 1. Gene-targeting exons 1 and 11 in the Oprm1 gene.

Figure 1

(A) Schematic of the targeting strategy. The coding and its adjacent intron regions of exons 1 and 11 were replaced with the tdTomato/BGHpolyA (tdT/pA)/PGK-Neo (neo) and ZsGree/SVpolyA (ZsG/pA) cassettes, respectively. The expected EcoRV-digested fragment lengths for WT and targeted alleles are indicated by arrows. The 5′ probe is indicated by a short line. LoxP and Flp sites are shown by triangles and diamonds, respectively. P, PI-SecI; E, EcoRV. (B) Southern blot analysis with the 5′ probe. (C) RT-PCR using RNAs from brain and appropriate primers. Each line represents data from 1 mouse. +/+, WT; +/–, heterozygous; –/–, homozygous.