Table II. Detergent selection for functional membrane proteomics. cmc, critical micelle concentration; AN, aggregation number; dtg, detergent; n/a, not available. DM, n-decyl-β-d-maltopyranoside; OG, n-octyl-β-d-glucoside. FA, steroid-based facial amphiphiles; FA-3, type 3 (2 maltosides); FA-4, type 4 (3 maltosides); FA-5, type 5 (3 glucosides). BP-1, beta-strand peptide type 1, (N) acetyl-(octyl)G-S-l-S-(N-methyl)l-d-(octyl)G-d-NH₂.

^a Source for cmc and aggregation number is Anatrace (A), Thermo Pierce (T), or specified reference.

^b Tissue-, cell- and protein-dependent, shown are general trends, based on Refs (6, 7, 24, 45, 49, 51–53, 61, 66–70), detergent charge state, count ratio over protein, PDB database, and experiences with CcO, hGPCR β₂AR and hLGIC GABA_AR. Utility/Compatibility: Extraction from membrane (E; success rate E > e; e: requiring sonication, or effective for mammalian and insect cells but less effective for bacterial cells), Keeping protein soluble (K), Activity maintenance (A; AA > Aa > A > a). Crystallography (X; X > x), EM (EM > em; em: often difficult to tell protein TM domains from detergent micelles), Digestion (D; D > d), and peptide RP HPLC MS (M; M > m). Unmarked means no or usually unconsidered.

^c After exhaustively replacing other detergents.

cmc, critical micelle concentration; AN, aggregation number; dtg, detergent; n/a, not available; DM, n-decyl-β-D-maltopyranoside; OG, n-octyl-β-D-glucoside; FA, steroid-based facial amphiphiles; FA-3, type 3 (2 maltosides); FA-4, type 4 (3 maltosides); FA-5, type 5 (3 glucosides); BP-1, beta-strand peptide type 1, (N) acetyl-(octyl)G-S-L-S-(N-methyl)L-D-(octyl)G-D-NH2.