Skip to main content
. 2015 Jul 27;125(8):3297–3302. doi: 10.1172/JCI79337

Figure 1. CASP1 activation by H. pylori depends on NLRP3, ASC, and TLR2.

Figure 1

(A–G) BMDCs from mice of the indicated genotypes were infected overnight with H. pylori NSH57, G27, and/or PMSS1. (A, C, and E) Western blot analysis of CASP1 activation (p10) in the cell supernatant compared to full-length CASP1 p45 and GAPDH in the extract. Representative results of 3 independent experiments are shown (n = 3). (B, D, and F) IL-1β ELISA of culture supernatants; cells were prestimulated with E. coli LPS prior to infection. Uninf, uninfected. (G) Il1b transcription, as measured by qRT-PCR (normalized to Gapdh and to uninfected controls). Mean + SD of 3 independent experiments is shown (n = 3). (H and I) CD11c+ splenic DCs were infected overnight with G27. (H) IL-1β and (I) IL-18 secretion was measured by ELISA. Representative data of 3 independent experiments are shown (n = 3). (J and K) Mice were infected for 1 month with PMSS1 prior to the quantification of (J) gastric colonization and (K) Ifng expression. Pooled data from 2 studies are shown (n = 2). Horizontal lines indicate medians. *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001, Mann-Whitney U test. Error bars represent mean + SD.