Figure 10. Prolonged inhibition of SNI-induced mechanical allodynia by i.t. BMSCs in the early or late phase and its reversal by TGF-β1 Abs.
(A and B) Long-term inhibition of mechanical allodynia by early treatment with BMSCs (2.5 × 105 cells) via i.t. injection, given 4 days after SNI. Mechanical allodynia was tested using the paw withdrawal threshold (A) and by the frequency of response (percentage of response) to a single 0.16 gf filament (10 times) (B). *P < 0.05, compared with vehicle (PBS); n = 6 mice/group. (C and D) Sustained inhibition of mechanical allodynia by late treatment with i.t. BMSCs (2.5 × 105 cells), given 21 days after SNI. *P < 0.05, compared with vehicle; n = 6 mice/group. (E) Reversal of BMSC-induced inhibition of mechanical allodynia by i.t. TGF-β1–neutralizing Abs (4 μg), given 3 weeks after SNI. Arrows in A–E indicate the time of injection. *P < 0.05, compared with the IgG control group; n = 5 mice/group. All data are expressed as the mean ± SEM. Statistical significance was determined by 2-way ANOVA, followed by Bonferroni’s post-hoc test.