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. 2015 Jul 20;125(8):3103–3116. doi: 10.1172/JCI80924

Figure 3. Cellular consequences of ELANE mutation: correction of ELANE mutation rescues granulocytic differentiation.

Figure 3

(A) Colony forming cell assay of the hematopoietic progenitors derived from ELANE-mutant SCN iPSCs (SCN15, SCN14) and the derivative ELANE mutation–corrected iPSCs (SCN15C, SCN14C). (B) Representative example of annexin V binding analysis in ELANE mutant– and CRISPR-corrected iPSC–derived granulocytic precursors. (C) Quantitation of the % annexin V binding. (D) Quantitation of granulopoietic differentiation of myeloid progenitors derived from SCN iPSCs (SCN15) and the corrected iPSCs (SCN15C). (E) Wright-Giemsa–stained cytospin at the end of the culture (SCN15, SCN15C). (F) Quantitation of granulopoietic differentiation of myeloid progenitors derived from SCN iPSCs (SCN14) and the corrected iPSCs (SCN14C). (G) Wright-Giemsa–stained cytospin at the end of the culture (SCN14, SCN14C). Data are presented as mean ± SD of 3 or 4 independent experiments.