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. 2015 Jul 20;125(8):3103–3116. doi: 10.1172/JCI80924

Figure 4. ER stress and NE mislocalization are hallmarks of ELANE-mutant iPSC–derived granulocytic precursors.

Figure 4

(A) qPCR analyses of the mRNA for UPR pathway genes BIP and ATF6 in RNA from control and SCN iPSC–derived promyelocytes. (B) Confocal microscopic analyses of the localization of NE and ER resident chaperone BIP in control and SCN iPSC–derived promyelocytes. Scale bars: 10 μm. (C) Quantitation of the correlation of colocalization coefficient between NE and BIP. (D) MFI of BIP measured from confocal microscopic images. In A, individual values of 2 or more independent experiments in duplicate are plotted as mean ± SD. In C and D, data from more than 10 cells from 2 independent experiments are presented as mean ± SD. Cont, control.