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. 2015 Jul 20;125(8):3103–3116. doi: 10.1172/JCI80924

Figure 5. Cytokine rescue of granulopoietic differentiation in myeloid precursors derived from SCN iPSCs.

Figure 5

(A) Quantitation of granulopoietic differentiation of myeloid progenitors derived from SCN iPSCs (SCN15) in liquid culture containing G-CSF at 50 ng/ml, 1,000 ng/ml, and 50 ng/ml, along with 230 nM sivelestat (a small-molecule inhibitor of NE). (B) Wright-Giemsa–stained cytospin at the end of the culture in A. (C) Quantitation of granulopoietic differentiation of myeloid progenitors derived from SCN iPSCs (SCN14) in liquid culture containing G-CSF at 50 ng/ml, 1,000 ng/ml, and 50 ng/ml, along with 230 nM sivelestat. (D) Wright-Giemsa–stained cytospin at the end of the culture in C. (E and F) qPCR analyses of the mRNA for CEBPB (E) and CEBPA (F) in promyelocytes derived from control or ELANE-mutant SCN iPSCs. In A and C, data from 4 independent experiments are plotted as mean ± SD. In E and F, individual data from 2 or more independent experiments with duplicates are plotted as mean ± SD. Scale bars: 10 μm. Cont, control.