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. 2015 Jul 13;125(8):3087–3102. doi: 10.1172/JCI81317

Figure 5. Age-dependent increases in adiposity in AnkB mutant mice and elevated lipid accumulation in differentiated adipocytes.

Figure 5

(A) Epididymal WAT sections from 3-month-old and 10-month-old mice stained for the lipid-associated protein perilipin. Scale bar: 20 μm. (B) Percentage of body fat mass. (C) Epididymal white adipose size (n = 6 mice). (D) Nonesterified free fatty acid (NEFA) levels (n = 10 mice). (E) Day 8 differentiated adipocytes stained for perilipin. Scale bar: 5 μm. (F) Lipid droplet volume in differentiated adipocytes (n = 100 cells for 1 of 3 independent experiments). Data represent mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001, 1-way ANOVA with post-hoc Tukey test.