Figure 6. Increased adiposity in Ankb^{R1788W/R1788W} mutant mice is not caused by major changes in appetite or activity.

(A and B) Representative images of congenic Ankb^+/+, Ankb^{L1622I/L1622I}, and Ankb^{R1788W/R1788W} (A) 3-month-old and (B) 10-month-old male mice. (C) Body weight. (D) Fat mass and (E) lean mass as a percentage of body weight. (F) Average 24-hour energy expenditure, (G) oxygen consumption, (H) CO₂ production measured by CLAMS. (I) Respiratory quotient. (J and L) Activity during a 24-hour period for (J) 3-month-old and (L) 10-month-old mice. (K and M) Food consumption on a regular diet during a 24-hour period for (K) 3-month-old and (M) 10-month-old mice. Data represent mean ± SEM, (n = 8, 3-month-old mice and n = 10, 10-month-old mice) for 1 experiment. *P < 0.05, **P < 0.01, ***P < 0.001, 1-way ANOVA with post-hoc Tukey test.