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. 2015 Jan 16;22(10):1563–1576. doi: 10.1038/cdd.2014.224

Figure 6.

Figure 6

HSD17B10 promotes mitochondrial elongation. (a) Representative immunocytochemical staining illustrating mitochondrial networks in COS7 cells transfected with siRNAs targeting the HSD17B10 gene or overproducing the protein. Mitochondria were visualized with anti-PMPCB and anti- HSD17B10 antibodies. Scale bar: 10 μm. (b) Quantitative confocal analysis of aspect ratio (length) and form factor (length and branching) of mitochondria in COS7 cells transfected as indicated. n=30 cells from three independent experiments. (c, d) Western blot analysis (c) and corresponding quantification (d) illustrating the effect of HSD17B10 downregulation or overproduction on the abundance of mitochondrial shaping proteins in COS7 cells. n=3 independent experiments. (e) Quantitative FRET analysis illustrating the effect of HSD17B10 overproduction on the molecular proximity between PINK1 and Drp1. n=15–20 cells from one experiment representative of three carried out. Results are expressed as means±S.E.M. ***P<0.001 versus ‘Empty vector+control siRNA' in (b); ‘control siRNA' in (d); and ‘- HSD17B10+CCCP' in (e)