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. 2015 Mar 6;22(10):1676–1686. doi: 10.1038/cdd.2015.16

Figure 2.

Figure 2

Functional inflammasomes in serum-deprived human neurons. (a) Ethidium bromide agarose gels of the NLRP1, NLRP3, IPAF-1, AIM2, and GAPDH RT-PCR amplicons from untreated and serum-deprived neurons compared with THP-1 cells. (b) NLRP1, IPAF-1, and AIM2 mRNA levels quantified relative to GAPDH mRNA levels by qRT-PCR in untreated and serum-deprived neurons. Statistical significance was assessed with a paired t-test. *P<0.05, **P<0.01. (c and d) Casp1 YVADase activity (c) and IL-1β (d) in S-100 cytosolic fractions isolated from serum-deprived primary human neurons (control) incubated without (untreated at 1.5 h 30 ºC) or with MDP, polydAT, and flagellin for 1.5 h at 30 ºC. (c and d) One-way ANOVA followed by Tukey–Kramer post hoc analysis (*P<0.05, **P<0.01) compares control with incubated samples, #P<0.05 and ##P<0.01 compares untreated with treated samples. Data represent mean and S.E.M. from three to six independent experiments (b–d)