Figure 5.

LPS-treated NLRP1^−/− and Casp1^−/− mice have reduced Casp1 and Casp6 activities. (a) Casp1-mediated IL-1β production, (b), Casp6 VEIDase activity (c), TubΔCasp6 western blots, (d) TubΔCasp6 relative to FL tubulin densitometry, and (f) Casp3 DEVDase activity in proteins extracted from the cortex of LPS-injected wild-type, NLRP1 null, and Casp1 null mice. Data represent the mean and S.E.M. from eight wild-type, six NLRP1 null, and three Casp1 null mice for (a,b and f), and three different mice for (c and d). Statistics were performed using one-way ANOVA (P=0.0014 for a, P=0.002 for b, and P=0.0001 for d) followed by a Tukey–Kramer post hoc test: **P<0.01, ***P<0.001 compares with WT control, ^###P<0.001 compares with WT LPS (a,b and d). (e) Western blots of full-length α-tubulin cleaved by Casp6 and Casp1