Fig. 6.

Attenuation of IL-6 and TNF-α production following apoptotic AGS co-incubation is not dependent on serum factors. Attenuation of IL-6 and TNF-α production following apoptotic AGS co-incubation is not dependent on serum factors. THP-1 macrophages were resuspended in serum free media (X-vivo10) or serum containing media (RPMI + 10 % v/v Non-Heat Inactivated FBS) and co-cultured with camptothecin (3 μM; 24 h) induced apoptotic AGS (CMPT AGS) or H. pylori (MOI 100; 24 h) infected AGS (Hp AGS) which were in the appropriate medium (THP-1: AGS ratio of 1:3). After 2 h co-incubation, apoptotic AGS were washed off with PBS. THP-1 macrophages were resuspended in the appropriate media (X-vivo 10 or RPMI + 10 % Non-heat inactivated FBS) and stimulated with LPS (100 ng/mL) or H. pylori (26695, MOI 100) for 24 h before collection of supernatants. Supernatants were centrifuged 10,000 × g for 10 min and stored at −80 °C until analyzed for IL-6 and TNF- α using specific ELISA. Data are shown as percent Control (THP-1 alone) of the relevant media condition from three independent experiments. Data comparisons between treatments performed in X-vivo 10 and RPMI + 10 % NHI FBS conditions were not significantly different