Specifications
Organism/cell line/tissue	Gallus gallus/primary embryonic cardiomyocytes/heart muscle
Sex	Mixed pool of male and female
Sequencer or array type	Illumina HiSeq 2000
Data format	Raw
Experimental factors	Mock-transfected versus siRNA-mediated knockdown of CELF1
Experimental features	Primary cardiomyocytes were isolated from chicken embryos at embryonic day 8 and transfected ± siRNA against CELF1. RNA-seq was performed to identify transcripts regulated by CELF1.
Consent	N/A
Sample source location	Fertilized chicken eggs were purchased from the Department of Animal Sciences at The Ohio State University in Columbus, OH. Eggs were shipped overnight via truck to the Cleveland Clinic Lerner Research Institute in Cleveland, OH, where eggs were incubated, cardiomyocytes were isolated, transfected, and cultured, and RNA was extracted. RNA samples were then shipped to The Ohio State University Comprehensive Cancer Center in Columbus, OH, where RNA-seq was performed.