Table 1.
Primers and plasmids used in this study.
| Primer | Sequence (5′ → 3′) | References |
|---|---|---|
| HR-F1 | TTAGGTACCGCTGTCCATTGCCTGA | This study |
| HS-R1 | AGACCAATCAGGAAATGCTCTGGACC | This study |
| HR-R1 | TATCTAGAGTCGCGTGCCAATACCAGTTC | This study |
| HR-F2.1 | AGGGTACCAGCGATATCCGTCAATTGACT | This study |
| HR-F2.2 | GAGGTACCTGTCTTGCCTGACAACGTTG | This study |
| HR-R2 | TATCTAGAATGTCATTTCTATGGAGGCATGAATCG | This study |
| HR-F3 | TATCTAGAGATGGTCAGCGCAGCG | This study |
| HS-F1 | GCAATCCTTTGCCGCAGCTATT | This study |
| HR-R3 | GTCAAGCTTCTAGGGCTCGTAGTTCG | This study |
| Plasmids | Description | References |
| pUC19 | High-copy cloning vector | Sigma |
| pRK767 | Low-copy cloning vector | Gill and Warren, 1988 |
| pUCF1 | LHR fragment 1 in pUC19 | This study |
| pUCF2 | LHR fragment 2 in pUC19 | This study |
| pUCF3 | LHR fragment 3 in pUC19 | This study |
| pUCF1-2 | LHR fragments 1-2 in pUC19 | This study |
| pRF1 | LHR fragment 1 in pRK767 | This study |
| pRF2 | LHR fragment 2 in pRK767 | This study |
| pRF3 | LHR fragment 3 in pRK767 | This study |
| pRF1-2 | LHR fragments F1-2 in pRK767 | This study |
| pLHR | The entire LHR sequence, F1-F2-F3, in pRK767 | This study |