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. 2015 Sep;45(11):673–678. doi: 10.1016/j.ijpara.2015.05.003

Fig. 2.

Fig. 2

Targets with differential expression patterns are susceptible to RNA interference (RNAi) in adult Ascaris suum, including those restricted to one tissue type. (A–C) Transcript reduction in a range of target tissues across head (a), gonopore (b) and tail (c) regions 3 days post-injection, including those with: (A) multi-tissue expression: GMP reductase (As-gmpr) knockdown (Aa) head: 78.5 ± 8.0%; (Ab) gonopore: 60.0 ± 15.7%; (Ac) tail: 96.4 ± 0.1%) in As-gmpr double-stranded RNA (dsRNA)-treated worms compared with zero dsRNA control worms (n = 6). (B) Neuronal expression: A. suum synaptic vesicle protein (As-rab-3) knockdown (Ba) head: 93.8 ± 2.5%; (Bb) gonopore: 85.4 ± 6.4%; (Bc) tail: 95.2 ± 1.4%) in As-rab-3-dsRNA treated worms compared with zero dsRNA control worms (n = 6); (C) Muscle eExpression: A. suum troponin C (As-tnc-1) knockdown (Ca) head: 80 ± 7.6%; (Cb) gonopore: 75.6 ± 9.8%; (Cc) tail: 92.1 ± 1.2%) in As-tnc-1-dsRNA-treated worms compared with zero dsRNA control worms (n = 6); in addition, knockdown was achieved for a gut and body wall expressed target (haemoglobin (As-hb-1; gonopore: 74.2 ± 11.2%; day 3, n = 3; P < 0.05, target dsRNA versus non-target dsRNA); graph not shown) and two nicotinic acetylcholine receptor subunits expressed in the neuromuscular system (As-unc-29, As-unc-38; see Fig. 3). Error bars represent S.E.M.; *P < 0.05, **P < 0.01.