Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2015 Sep;45(11):673–678. doi: 10.1016/j.ijpara.2015.05.003

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2015 The Authors

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Fig. 3 — RNA interference (RNAi) is achievable using a ‘multi-target dsRNA cocktail’ delivery approach and in different geographical isolates of Ascaris suum. (A) Two nicotinic acetylcholine receptor subunits, As-unc-29 and As-unc-38, transcript knockdown (8 days post injection) in gonopore-tissue segments of adult A. suum collected in Ballymena, Northern Ireland (European Isolate: As-unc-29 knockdown in target dsRNA-treated worms compared with zero dsRNA controls is 92.9 ± 1.8% (n = 6); As-unc-38 knockdown in target dsRNA-treated worms compared with zero dsRNA controls is 83.9 ± 3.3% (n = 6)). (B) As-unc-29 and As-unc-38 transcript knockdown (8 days post-injection) in gonopore tissue segments of adult A. suum collected in Marshalltown, Iowa, U.S.A. (North American Isolate: As-unc-29 knockdown in target dsRNA-treated worms compared with zero dsRNA controls is 84.2 ± 3.4% (n = 6); As-unc-38 knockdown in target dsRNA-treated worms compared with zero dsRNA controls is 77.1 ± 4.5% (n = 6)). Note that transcript knockdown was also assessed in the head region (European isolate only: As-unc-29 knockdown in target dsRNA-treated worms compared with zero dsRNA controls is 86.3 ± 7.7% (n = 5); P < 0.05, target dsRNA versus non-target dsRNA; As-unc-38 knockdown in target dsRNA-treated worms compared with zero dsRNA controls is 83.9 ± 7.4% (n = 5); P < 0.05, target dsRNA versus non-target dsRNA; graphs not shown), and muscle bag cells (North American isolate only: As-unc-29 knockdown in target dsRNA-treated worms compared with zero dsRNA controls is 82.2 ± 6.2% (n = 6); P < 0.05, target dsRNA versus non-target dsRNA; As-unc-38 knockdown in target dsRNA-treated worms compared with zero dsRNA controls is 85.6 ± 3.8% (n = 6); P < 0.05, target dsRNA versus non-target dsRNA; graphs not shown). Error bars represent S.E.M; ^*P < 0.05, ^**P < 0.01. Note that in all cases target dsRNA-treated worms were injected with a cocktail of dsRNA-As-unc-29 and dsRNA-As-unc-38.