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. 2015 Aug 5;36(4):947–956. doi: 10.3892/ijmm.2015.2304

Figure 2.

Figure 2

Cyclic mechanical stretch activates the Akt/mammalian target of rapamycin (mTOR)/p70s6k pathway in osteoblast-like MG-63 cells. (A) Western blot analysis of the stretch-induced protein expression of Akt, phosphorylated Akt (p-Akt on Thr308 and Ser473), mTOR, phosphorylated mTOR (p-mTOR, Ser2448), p70s6k, and phosphorylated p70s6k (p-p70s6k, Thr389). (B) Immunofluorescence staining of stretch-induced protein expression of Akt, phosphorylated Akt (p-Akt, Thr308), mTOR, and phosphorylated mTOR (p-mTOR, Ser2448) (scale bar, 50 µm). Data are presented as the means ± SD (n=3). *P<0.05 and **P<0.01. Ctrl, control.