Figure 5.
Function analysis of ubiquitin-associated protein 2-like (UBAP2L) by short hairpin RNA (shRNA) in A172 cells. (A) Representative images of A172 cells after 4 days of lentivirus infection are shown. (B) Reverse transcription-quantitative polymerase chain reaction analysis of UBAP2L mRNA levels in A172 cells. β-actin was the internal control. (C) Cell proliferation in Lv-shUBAP2L groups was significantly inhibited, as detected by the MTT assay. (D and E) Downregulated UBAP2L induced cell cycle arrest at S phase in A172 cells. Scale bar, 100 µm. ***P<0.001.