Figure 4.

Shen-Kang (SK) pre-treatment inhibits the H₂O₂-induced apoptosis of HK-2 cells. (A) Apoptosis of HK-2 cells was detected by flow cytometry with Annexin V and propidium iodide (PI) staining. Cells were treated for 24 h with 500 μM H₂O₂ or 300 μg/ml SK followed by 500 μM H₂O₂. (B) Examination of HK-2 cell nuclear morphology by fluorescence microscopy after staining with 4′,6-diamidino-2-phenylindole (DAPI). (C) Western blot analysis of cleaved poly(ADP-ribose) polymerase (PARP) following treatment with 500 μM H₂O₂ and/or 300 μg/ml SK in HK-2 cells. Values are expressed as the means ± SEM of 3 individual experiments. ^**P<0.01 compared with the control; ^#P<0.05 compared with the H₂O₂-treated group.