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. Author manuscript; available in PMC: 2016 Sep 1.
Published in final edited form as: Cell Mol Bioeng. 2015 Sep;8(3):320–332. doi: 10.1007/s12195-015-0413-8

Figure 2.

Figure 2

Protocol of hiPSC cardiac differentiation. (A) Timeline and representative images of the monolayer-based cardiac differentiation protocol and plating regimen for these studies. The timing and duration of specific morphogens (Activin-A and Bone Morphogenic Protein-4 [BMP4]) and small molecule Wnt modulators (Chiron and Xav939) specify cardiomyocyte development. (B) Representative flow cytometry result of cardiac purity for a cardiac differentiation prep. Differentiation runs with cardiac purity >70% were used for subsequent analysis.