Fig. 1.

Mating type and fumonisin-producing capability in F. verticilioides strains of Texas. (A) PCR of the mating-type loci genes and FUM1 gene in 164 F. verticillioides field isolates and reference strains (7600 and 7598). Results were analyzed by electrophoresis on a 1.0% agarose gel stained with ethidium bromide. Lane 1, Wild-type strain 7600 (MAT1-1); Lane 2, Wild-type strain 7598 (MAT1-2); Lane 3–7, isolates Victoria #1, Waller #2, Falls #38, Medina #240A and Robertson #242A, respectively. (B) Isolate mating type was color-coded and graphed onto a Texas map based on the county of origin for all isolates. Counties colored blue and red represent counties where all samples isolated were MAT1-1 and MAT1-2, respectively. Green counties are those where mixed mating-type populations were observed.